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Cell Cycle Regulatory Gene Study in Patients With Myeloproliferative Disorders

Descriptive Information
Brief Title † Cell Cycle Regulatory Gene Study in Patients With Myeloproliferative Disorders
Official Title † Cell Cycle Regulatory Gene Study in Patients With Myeloproliferative Disorders
Brief Summary This study involves observing the level of cell cycle regulatory gene in patients with myeloproliferative disorders(MPD). These disorders include polycythemia vera (PV), essential thrombocythemia (ET), myelofibrosis (MF) and chronic myeloid leukemia (CML). The abnormal blood and/or bone marrow cells, or materials derived from these abnormal cells, like DNA, RNA, protein or plasma will be used in laboratory studies. Cell cycle regulatory protein such as cyclins, cyclin-dependent kinases(Cdks) and Cdk inhibitors(CKIs) play indispensable roles in processes such as transcription, metabolism and stem cell self-renewal. MPD are a group of diseases characterized by abnormally increased proliferation of erythroid, megakaryocytic, or granulocytic cells. The pathogenesis was still unclear. Detecting the level of cell cycle regulatory protein will be useful to look for the possible role in MPD and better understand the cause of MPD.
Detailed Description This study involves observing the level of cell cycle regulatory gene in patients with myeloproliferative disorders(MPD). These disorders include polycythemia vera (PV), essential thrombocythemia (ET), myelofibrosis (MF) and chronic myeloid leukemia (CML). The abnormal blood and/or bone marrow cells, or materials derived from these abnormal cells, like DNA, RNA, protein or plasma will be used in laboratory studies. Cell cycle regulatory protein such as cyclins, cyclin-dependent kinases(Cdks) and Cdk inhibitors(CKIs) play indispensable roles in processes such as transcription, metabolism and stem cell self-renewal. MPD are a group of diseases characterized by abnormally increased proliferation of erythroid, megakaryocytic, or granulocytic cells. The pathogenesis was still unclear. Detecting the level of cell cycle regulatory protein will be useful to look for the possible role in MPD and better understand the cause of MPD. In this study, the cell cycle regulatory message RNA and protein from patients' and healthy volunteers' bone marrow and peripheral blood will be extracted and quantified by real time polymerase chain reaction and western blot. The different level of cell cycle regulatory gene between patients and volunteers and the relationship between the level and patients' clinical characteristics such as age, gender, type of disease will be analysed by SPSS.
Study Phase N/A
Study Type † Observational
Study Design † Observational Model: Case Control, Time Perspective: Prospective
Primary Outcome Measure † quantified the level of CDK, CCP, PLK by real time PCR and westen blot
Secondary Outcome Measure †
Condition † Myeloproliferative Disorders Polycythemia Vera Essential Thrombocythemia Myelofibrosis Chronic Myeloid Leukemia
Intervention †
Study Arms / Comparison Groups
Publications *

* Includes publications given by the data provider as well as publications identified by National Clinical Trials Identifier (NCT ID) in Medline.

Recruitment Information
Recruitment Status †
Estimated Enrollment † 0
Start Date † June 2016
Completion Date June 2020
Primary Completion Date June 2018
Eligibility Criteria † Inclusion Criteria: - Have a diagnosis of polycythemia vera (PV), essential thrombocythemia (ET), myelofibrosis (MF) or chronic myeloid leukemia (CML) as defined by the World Health Organization (WHO) diagnostic criteria Exclusion Criteria: - Have received treatment
Gender Both
Ages 18 Years - 80 Years
Accepts Healthy Volunteers Accepts Healthy Volunteers
Contacts ††
Location Countries † China
Administrative Information
NCT ID † NCT02663648
Organization ID Cell cycle regulatory gene-MPD
Secondary IDs ††
Responsible Party Principal Investigator
Study Sponsor † Shandong University
Collaborators ††
Investigators † Principal Investigator: Jun Peng, Qilu hospital, Shandong University
Information Provided By
Verification Date January 2016
First Received Date † December 21, 2015
Last Updated Date April 18, 2016
† Required WHO trial registration data element.
†† WHO trial registration data element that is required only if it exists.
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