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Synthesis of glycocluster-containing conjugates for a vaccine against cholera.
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Synthesis of glycocluster-containing conjugates for a vaccine against cholera.

Org Biomol Chem. 2019 Apr 05;:

Authors: Pfister HB, Kelly M, Qadri F, Ryan ET, Kováč P

Abstract
Glycoclusters displaying synthetic fragments of the O-specific polysaccharide (OSP) of Vibrio cholerae O1 serotype Inaba on a carbohydrate platform were prepared by Cu(i)-catalysed azide alkyne cycloaddition (CuAAC, click chemistry). The clusters were subsequently conjugated to BSA via squaric acid chemistry. Their immunoreactivity was compared with those of similar conventional conjugates, i.e. made from single oligosaccharides presented in non cluster form, using plasma of patients recovering from cholera. The results showed that the conjugates were displayed in immunologically relevant manners and that the immunoreactivity of hexasaccharide-cluster conjugates was similar to that of a conjugate displaying OSP isolated from wild type V. cholerae, further supporting the immunologic relevance of antigens made from synthetic oligosaccharides.

PMID: 30950473 [PubMed - as supplied by publisher]

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