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Related Articles

Inflammation-induced down-regulation of butyrate uptake and oxidation is not caused by a reduced gene expression.

J Cell Physiol. 2014 Jul 24;

Authors: Boesmans L, Ramakers M, Arijs I, Windey K, Vanhove W, Schuit F, Rutgeerts P, Verbeke K, Preter VD

Abstract
In ulcerative colitis (UC) the butyrate metabolism is impaired, leading to energy-deficiency in the colonic cells. The effect of inflammation on the butyrate metabolism was investigated. HT-29 cells were incubated with pro-inflammatory cytokines (TNF-α and/or IFN-γ) for 1h and 24h. Cells were additionally stimulated with butyrate to investigate its anti-inflammatory potential. Butyrate uptake and oxidation were measured using (14) C-labelled butyrate. Gene expression of the butyrate metabolism enzymes, interleukin 8 (IL-8; inflammatory marker) and villin-1 (VIL-1; epithelial cell damage marker) was measured via quantitative RT-PCR. Significantly increased IL-8 expression and decreased VIL-1 expression after 24h incubation with TNF-α and/or IFN-γ confirmed the presence of inflammation. These conditions induced a decrease of both butyrate uptake and oxidation, whereas the gene expression was not reduced. Simultaneous incubation with butyrate counteracted the reduced butyrate oxidation. In contrast, 1h incubation with TNF-α induced a significant increased IL-8 expression and decreased butyrate uptake. Incubation with TNF-α and/or IFN-γ for 1h did not induce cell damage nor influence butyrate oxidation. The inflammation-induced down-regulation of the butyrate metabolism was not caused by a reduced gene expression, but appeared consequential to a decreased butyrate uptake. Increasing the luminal butyrate levels might have therapeutic potential in UC. © 2014 Wiley Periodicals, Inc.

PMID: 25059646 [PubMed - as supplied by publisher]

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